Disruption of microbial cells for intracellular products

Disruption of microbial cells for intracellular products YUSUF CHISTI and MURRAY MOO-YOUNG* Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G I Summary. Disintegration of microbial cells is a necessary first step for the production of intracellular enzymes and organelIes.

• Published in: Enzyme and Microbial Technology · 1986Authors: Yusuf Chisti · Murray MooyoungAffiliation: University of WaterlooAbout: Bacteria · Microorganism · Yeast · Enzymeاحصل على السعر >>

Cell Disruption - APV Hemisan

used for cell disruption and reported on the relative efficiencies of the various techniques. By 1971, Jakoby19 indicated that the two devices used for large-scale cell disruption were the bead mill and the high-pressure homogenizer. Follow, et al.20 and Hetherington, et al.21 reported on detailed studies using

Influence of processing parameters on disintegration of ...

Aug 29, 2008 · While the degree of the cell disruption decreased with increasing cell density in Dyno-Mill and LabStar, the cell disruption in the MS 18 increased. Depending on processing parameters, more than 90% of algae cells were disrupted by passing through the bead mills and bacteria count in algae suspension was reduced to about two orders.

Cell Disruption - APV Hemisan

used for cell disruption and reported on the relative efficiencies of the various techniques. By 1971, Jakoby19 indicated that the two devices used for large-scale cell disruption were the bead mill and the high-pressure homogenizer. Follow, et al.20 and Hetherington, et al.21 reported on detailed studies using

Ultrasonic cell disruption - London South Bank University

Ultrasonic cell disruption. The treatment of microbial cells in suspension with inaudible ultrasound (greater than about 18 kHz) results in their inactivation and disruption. Ultrasonication utilises the rapid sinusoidal movement of a probe within the liquid.

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Cell Disruption - APV Hemisan

used for cell disruption and reported on the relative efficiencies of the various techniques. By 1971, Jakoby19 indicated that the two devices used for large-scale cell disruption were the bead mill and the high-pressure homogenizer. Follow, et al.20 and Hetherington, et al.21 reported on detailed studies using

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Influence of processing parameters on disintegration of ...

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Comparative study of fungal cell disruption—scope and ...

Sep 08, 2011 · Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis.Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, application of chemicals of various types, and osmotic shock.

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Yeast cell disruption with Retsch Mixer Mill MM 400 - YouTube

Jul 16, 2015 · RETSCH's Mixer Mill MM 400 is a true purpose mill: It is suitable for mixing, and homogenizing powders and suspensions within seconds and is also used for disruption of biological cells .

Cell disruption - Wikipedia

It has since been used in other applications such as cell disruption nanoemulsions, and solid particle size reduction, among others. By using microchannels with fixed geometry, and an intensifier pump, high shear rates are generated that rupture the cells. This method of cell lysis can yield breakage of over 90% of E. coli cells.

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The usefulness of the Netzsch LME 20-mill for the disruption of bacteria species was demonstrated with Escherichia coli, Brevibacterium ammoniagenes, Bacillus sphaericus and Lactobacillus confusus. As expected, the mill capacity for bacterial disruption was significantly smaller than for the yeast.

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